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CAS RN: 29270-56-2 | Product Number: A5593

NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

Purity: >99.0%(HPLC)
  • 4-Fluoro-7-nitrobenzofurazan
  • 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
18   9   ≥20 

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Product Number A5593
Purity / Analysis Method >99.0%(HPLC)
Molecular Formula / Molecular Weight C__6H__2FN__3O__3 = 183.10  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive,Heat Sensitive
CAS RN 29270-56-2
Reaxys Registry Number 1077571
PubChem Substance ID 87563200
SDBS (AIST Spectral DB) 50330
MDL Number


Appearance White to Light yellow to Green powder to crystal
Purity(HPLC) min. 99.0 area%
Melting point 53.0 to 57.0 °C
Effect test of HPLC derivatization Effective as labeling agent for L-Isoleucine
Properties (reference)
Melting Point 55 °C
Solubility (soluble in) Chloroform
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 2934.99.4400
HPLC Labeling Reagent for Amines and Thiols

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with a secondary amine and thiol. The resultant derivative is stable enough to reach the detector without any decomposition under general reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 46 nm and 535 nm, respectively. Application example:[Alkylamines] 1)To 25~5 μL of a methanol solution containing an amine (1~2 μg), 4~8 eq. excess amount of .5% labeling reagent 1 / methanol solution is added. After adding 5~1 μL of .1 M NaHCO3, incubate at 55 ºC for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.[Others]TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals2,3) Hydrolyze the carbamates to label the amine derivatives.TLC of amphetamines in urine4,5), HPLC of prolines (precolumn derivatization method)6)TCI Products Pamphlet


1)K. Imai, Y. Watanabe, Anal. Chim. Acta 1981, 130, 377.

2)Y. Watanabe, K. Imai, Anal. Biochem. 1981, 116, 471.

3)Y. Watanabe, K. Imai, J. Chromatogr. 1982, 239, 723.

4)T. Toyo’oka, Y. Watanabe, K. Imai, Anal. Chim. Acta 1983, 149, 305.

5)Y. Watanabe, K. Imai, Anal. Chem. 1983, 55, 1786.

6)Y. Watanabe, K. Imai, J. Chromatogr. 1984, 309, 279.

7)H. Miyano, T. Toyo’oka, K. Imai, Anal. Chim. Acta 1985, 170, 81.

8)H. Kotaniguchi, M. Kawakatsu, T. Toyo’oka, K. Imai, J. Chromatogr. 1987, 420, 141.

PubMed Literature


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