CAS RN: | Product Number: A2968
Anti-Keratan Sulfate Monoclonal Antibody (R-10G)
|Size||Unit Price||Philadelphia, PA||Portland, OR||Japan*||Quantity|
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Volume/Quantity : 0.1 mg
Product Form : Purified mouse monoclonal antibody/IgG1
Buffer : Phosphate buffered-saline (PBS)
Preparation : The antibody is purified from mouse ascites fluid.
Preservative : 0.05% Sodium azide
Stabilizer : None
Immunogen : Human iPS cell (Tic)
Specificity : Keratan Sulfate
Clone Name : R-10G
The R-10G monoclonal antibody specifically binds to human induced pluripotent stem cells(hiPS) and human embryonic stem cells(hES), is useful tool for the evaluation of hiPS/hES cells. The epitope of R-10G is a unique form of the keratan sulfate associated with podocalyxin on hiPS/ES cells, which lacks oversulfated structures. The epitope is also expressed in the adult human and mouse brains.
Flow cytometry, Immunoprecipitation, Immunoaffinity chromatography, ELISAWestern Blotting, Immunohistochemistry
Store at －20 ºC to －80 ºC (Preferably at －80 ºC). It is not recommended storing at 4 ºC. Avoid repeated freezing and thawing.
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|Physical State (20 deg.C)||Liquid|
|Appearance||Colorless to Almost colorless clear liquid|
|Titer(ELISA)||to pass test|
A novel antibody for human-induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures
K. Kawabe, D. Tateyama, H. Toyoda, N. Kawasaki, N. Hashii, H. Nakao, S. Matsumoto, M. Nonaka, H. Matsumura, Y. Hirose, A. Morita, M. Katayama, M. Sakuma, N. Kawasaki, M. K. Furue, T. Kawasaki, Glycobiology 2013, 23, 322-336.
A cytotoxic antibody recognizing lacto-N-fucopentaose I (LNFP I) on human induced pluripotent stem (hiPS) cells
S. Matsumoto, H. Nakao, K. Kawabe, M. Nonaka, H. Toyoda, Y. Takishima, K. Kawabata, T. Yamaguchi, M. K. Furue, T. Taki, T. Okumura, Y. Yamazaki, S. Nakaya, N. Kawasaki, T. Kawasaki, J. Biol. Chem. 2015, 290, 20071-20085.
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