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CAS RN: | Product Number: A2849

Anti-Sialyl Lewis X Monoclonal Antibody


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1VIAL
$908.00
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Product Information
Volume/Quantity :  0.1 mg/vial
Product Form : Purified mouse monoclonal antibody/IgM, κ-chain
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preparation : The antibody is purified from serum-free cell culture supernatant by column chromatography.
Preservative : 0.05% sodium azide
Stabilizer : None
Immunogen : A mixture of sialyl Lewis X-like determinants from human colon carcinoma cells
Specificity : Sialyl Lewis X
Clone Name : 2H5

Application
Immunohistochemistry, flow-cytometric analysis, ELISA, Western blotting,cell adhesion inhibition experiments

Storage
Store at -20 °C to -80 °C (Preferably at -80 °C). It is not recommended storing at 4 °C. Avoid repeated freezing and thawing.

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Product Number A2849
Physical State (20 deg.C) Liquid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorless clear liquid
Titer(ELISA) to pass test
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3504.00.5000
Application
Anti-Sialyl Lewis X Monoclonal Antibody
References

Specific expression of a complex sialyl Lewis X antigen on high endothelial venules of human lymph nodes: Possible candidate for L-selectin ligand.

M. Sawada, A. Takada, I. Ohwaki, N. Takahashi, H. Tateno, J. Sakamoto, R. Kannagi, Biochem. Biophys. Res. Commun. 1993, 193, 337-347.

Sulfated sialyl Lewis X, the putative L-selectin ligand, detected on endothelial cells of high endothelial venules by a distinct set of anti-sialyl Lewis X antibodies.

C. Mitsuoka, N. Kawakami-Kimura, M. Kasugai-Sawada, N. Hiraiwa, K. Toda, H. Ishida, M. Kiso, A. Hasegawa, R. Kannagi, Biochem. Biophys. Res. Commun. 1997, 230, 546-551.

Identification of a major carbohydrate capping group of the L-selectin ligand on high endothelial venules in human lymph nodes as 6-sulfo sialyl Lewis X.

C. Mitsuoka, M. Sawada-Kasugai, K. Ando-Furui, M. Izawa, H. Nakanishi, S. Nakamura, H. Ishida, M. Kiso, R. Kannagi, J. Biol. Chem. 1998, 273, 11225-11233.

L-Selectin ligands that are O-glycoprotease resistant and distinct from MECA-79 antigen are sufficient for tethering and rolling of lymphocytes on human high endothelial venules.

R. A. Clark, R. C. Fuhlbrigge, T. A. Springer, J. Cell Biol. 1998, 140, 721-731.

Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by co-transfection of α1→3 fucosyltransferase VII and newly cloned GlcNAcβ: 6-sulfotransferase cDNA.

N. Kimura, C. Mitsuoka, A. Kanamori, N. Hiraiwa, K. Uchimura, T. Muramatsu, T. Tamatani, G. S. Kansas, R. Kannagi, Proc. Natl. Acad. Sci. USA 1999, 96, 4530-4535.

Sialyl LewisX (CD15s) monitoring as a means to select antirejection therapy in patients with rejection after renal transplantation: CD15s monitoring for treatment and diagnosis in patients with acute rejection after renal transplantation

I H. Ishida, I. Koyama, T. Sawada, T. Murakami, T. Tojimbara, I. Nakajima, K. Tanabe, Y. Yamaguchi, S. Fuchinoue, H. Toma, T. Agishi, Transplantation 2000, 69, 2208-2211.

High endothelial venule-like vessels in the interstitial lesions of human glomerulonephritis

M. Takaeda, H. Yokoyama, C. Segawa-Takaeda, T. Wada, K. Kobayashi, Am. J. Nephrol. 2002, 22, 48-57.

Contribution of selectin ligands to eosinophil recruitment into the skin of patients with atopic dermatitis

T. Satoh, M. Kaneko, M.-H. Wu, H. Yokozeki, K. Nishioka, Eur. J. Immunol. 2002, 32, 1274-1281.

Anti-oligosaccharide antibodies as tools for studying sulfated sialoglycoconjugate ligands for siglecs and selectins

R. Kannagi, K. Ohmori, N. Kimura, Glycoconj. J. 2009, 26, 923-928.


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