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Redox Coenzymes

Enzymatic cycling methods have been designed for amplifying sensitivity to assay a small amount of substrate or enzyme activity.1) Earlier studies on enzymatic cycling methods were described on DAN+, NADP+, and CoA.2-4) Thio-NAD+ (1) contributed to improvement of the enzymatic cycling method.1,5)
Conversion of MVA to HMG-CoA by HMG-CoA reductase

Conversion of MVA to HMG-CoA by HMG-CoA reductase6)

An example of application of 1 for the enzymatic cycling method is shown above. As 1 is converted to thio-NADH (2) by such an enzymatic reaction, the reaction can be traced by the production of 2. Accumulation of 2 can be monitored by the increase in its absorbance (400 nm), which is at a longer wavelength than NADH (340nm).8) On the other hand, 2 was also applied for analysis of the active form of bleomycin.9)


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