CAS RN: | Product Number: T3531
Streptavidin Maleimide Conjugate
|Size||Unit Price||Same Day||2-3 Business Days|
Conjugation:Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form:Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Specific Activity:>10 U/mg , HABA dye-binding method
Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.
1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 ºC or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.
Store at －20 ºC to －80 ºC (Preferably at －80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
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|Appearance||White to Brown to Dark green powder to crystal|
|Specific Activity||to pass test|
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