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CAS RN: | Product Number: T3531

Streptavidin Maleimide Conjugate

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Product Information
Volume/Quantity:0.5 mg
Conjugation:Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form:Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Specific Activity:>10 U/mg , HABA dye-binding method

Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.

1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 ºC or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.

Store at -20 ºC to -80 ºC (Preferably at -80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

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Product Number T3531
Physical State (20 deg.C) Solid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
PubChem Substance ID 354335029
Appearance White to Brown to Dark green powder to crystal
Specific Activity to pass test
Properties (reference)
Related Laws:
Transport Information:
H.S.code* 3504.00-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
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