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CAS RN: 189373-41-9 | Product Number: A5574

DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]

  • 4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole
3   1  

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Product Number A5574
Molecular Formula / Molecular Weight C__1__0H__1__5N__5O__3S = 285.32  
Physical State (20 deg.C) Solid
CAS RN 189373-41-9
Reaxys Registry Number 18820975
PubChem Substance ID 87563191
MDL Number


Appearance Light yellow to Brown powder to crystal
Elemental analysis(Nitrogen) 23.30 to 25.80 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid
Properties (reference)
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H.S.code* 2935.90-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC labeling reagent, which has a bromoacetyl group and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection. Application examples:[Fatty acids]1, 2, 8, 9)Dissolve a sample in methanol or water, and then neutralize the sample solution with methanol solution of KOH-crown ether. Evaporate to dryness under reduced pressure, and then you will see a generally almost white solid substance remaining (potassium salt of fatty acid). Next, add the HPLC labeling reagent 1 with acetonitrile solution* of 18-crown 6-ether to this white solid and further add acetonitrile for a volume up to 1 mL. Incubate the solution at 8 ºC for 15 min. Cool the resultant solution to room temperature and use it as an HPLC sample.* Benzene can be used in the place of acetonitrile. The mixing ratio (molar ratio) for the HPLC labeling reagent 1 and 18-crown 6-ether should be 2 to 1 and 1 to 1 for the sample fatty acid concentrations at .5~2 mM and less than .5 mM, respectively. Use the excessive amount of the reagent 1.[Others]Dicarboxyl acids2), synthetic prostaglandins3), unsaturated fatty acids4), alkyl methylphosphonate5), ganglioside6), betaine7)TCI Products Pamphlet


1)Tokyo Kasei Kogyo, Jpn. Kokai Tokkyo Koho 98 218871, 1998.

2)P. Prados, T. Fukushima, T. Santa, H. Homma, M. Tsunoda, S. Al-Kindy, S. Mori, H. Yokosu, K. Imai, Anal. Chim. Acta 1997, 344, 227.

PubMed Literature


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