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CAS RN: 28718-90-3 | Product Number: A2412

2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride [for Biochemical Research]


Purity: >90.0%(HPLC)
Synonyms:
  • 4',6-Diamidino-2-phenylindole Dihydrochloride
  • DAPI·2HCl
  • 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
Documents:
5MG
$48.00
4   3  

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* The storage conditions are subject to change without notice.


Product Number A2412
Purity / Analysis Method >90.0%(HPLC)
Molecular Formula / Molecular Weight C__1__6H__1__5N__5·2HCl = 350.25  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 28718-90-3
Reaxys Registry Number 4894417
PubChem Substance ID 135727109
MDL Number

MFCD00012681

Specifications
Appearance Light yellow to Yellow to Orange powder to crystal
Purity(HPLC) min. 90.0 area%
DNA staining test to pass test
Properties (reference)
GHS
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Transport Information:
H.S.code* 2933.99-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Application
Fluorescence Staining of Cell Nucleus

DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.

A2412

HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.

References


PubMed Literature


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