CAS RN: 68123-33-1 | Product Number: A5522
N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]
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|Purity / Analysis Method||>98.0%(N)|
|Molecular Formula / Molecular Weight||C__1__2H__1__0N__2O__6 = 278.22|
|Physical State (20 deg.C)||Solid|
|Store Under Inert Gas||Store under inert gas|
|Condition to Avoid||Moisture Sensitive,Heat Sensitive|
|Reaxys Registry Number||6228910|
|PubChem Substance ID||87563163|
|SDBS (AIST Spectral DB)||11057|
|Appearance||White to Almost white powder to crystal|
|Purity(with Total Nitrogen)||min. 98.0 %|
|Melting point||181.0 to 185.0 °C|
|Solubility in hot Acetone||almost transparency|
|Effect test of HPLC derivatization||Effective as labeling agent for Ｄiethylamine.|
|Melting Point||181 °C|
|Hazard Statements||H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
|Precautionary Statements||P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
The compound 1 easily reacts with an amino group to form the corresponding 2,4-dinitrophenylamine derivative. The resultant derivative is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection. Application examples:[Amines]A sample (free amine) 1 mg, chloroform 1 mL, and labeling regent 1 (1 eq. excess amount of the sample) are mixed, and incubated at 6 ºC for 1 h. After cooling to room temperature, use it as an HPLC sample. 1 is also used for derivatization of amino acids.1,2)[Others]Aminoglycosides3)TCI Products Pamphlet
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