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CAS RN: 68123-33-1 | Product Number: A5522

N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]

Purity: >98.0%(N)
  • N-(4-Nitrophenylacetoxy)succinimide
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Product Number A5522
Purity / Analysis Method >98.0%(N)
Molecular Formula / Molecular Weight C__1__2H__1__0N__2O__6 = 278.22  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Moisture Sensitive,Heat Sensitive
CAS RN 68123-33-1
Reaxys Registry Number 6228910
PubChem Substance ID 87563163
SDBS (AIST Spectral DB) 11057
MDL Number


Appearance White to Almost white powder to crystal
Purity(with Total Nitrogen) min. 98.0 %
Melting point 181.0 to 185.0 °C
Solubility in hot Acetone almost transparency
Effect test of HPLC derivatization Effective as labeling agent for Diethylamine.
Properties (reference)
Melting Point 181 °C
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws:
EC Number 268-551-1
Transport Information:
HS Number 2928009090
HPLC Labeling Reagent for Amines

The compound 1 easily reacts with an amino group to form the corresponding 2,4-dinitrophenylamine derivative. The resultant derivative is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection. Application examples:[Amines]A sample (free amine) 1 mg, chloroform 1 mL, and labeling regent 1 (1 eq. excess amount of the sample) are mixed, and incubated at 6 ºC for 1 h. After cooling to room temperature, use it as an HPLC sample. 1 is also used for derivatization of amino acids.1,2)[Others]Aminoglycosides3)TCI Products Pamphlet


1)T. H. Jupille, Am. Lab. 1976, 8, 85.

PubMed Literature

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