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CAS RN: 601494-52-4 | 产品编码: A2083

ATBTA-Eu3+


纯度/分析方法: >90.0%(T)(HPLC)
别名:
  • ATBTA-Eu3+
  • [4'-(4'-氨基-4-联苯基)-2,2':6',2''-三联吡啶-6,6''-二基双(甲基亚氨基二乙酸根)]铕(III)酸钠
  • Sodium [4'-(4'-Amino-4-biphenylyl)-2,2':6',2''-terpyridine-6,6''-diylbis(methyliminodiacetato)]europate(III)
文档:
10MG
¥ 790.00
7   1   ≥20 
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产品编码 A2083
纯度/分析方法 >90.0%(T)(HPLC)
分子式/分子量 C__3__7H__3__0EuN__6NaO__8 = 861.63  
外观与形状(20°C) 固体
储存在惰性气体下 存放于惰性气体之中
应避免的情况 光,空气
CAS RN 601494-52-4
PubChem物质ID 87560385
技术规格
Appearance Light yellow to Brown to Dark green powder to crystaline
Purity(HPLC) min. 90.0 area%
Purity(Chelometric Titration) min. 90.0 %
Water max. 10.0 %
物性(参考值)
水溶性 可溶
GHS
相关法规
运输信息
监管条件代码(*)
应用
Europium Fluorescent Labeling Reagent suitable for Time-resolved Fluorometry

 ATBTA-Eu3+ is a europium chelate complex and can be used as a fluorescent labeling reagent. ATBTA-Eu3+ is easily labeled to protein etc. after conversion to DTBTA-Eu3+.

A2083

 The new lanthanide chelate reagent, DTBTA-Eu3+, has a high stability constant, and therefore the problem of fluorescence intensity change in different buffers has been greatly reduced. DTBTA-Eu3+ has also several advantages such as the intensity stability in water for a long period, and the stability against photo-bleaching.

Typical Procedure: Preparation of DTBTA-Eu3+
 Dissolve 2mg of ATBTA-Eu3+ [A2083] in 60µL of 0.1M acetate buffer (pH4.9). This solution is added 0.43mg of Cyanuric Chloride [C0460] in 25µL of acetone, and stirred for 30 min. The reaction mixture is added dropwise to 1mL of acetone, and formed precipitate is centrifuged. After washing with 0.5mL of acetone twice, the yellow powder is dried in vacuum for 1 h. Dissolve the powder in 1mL of carbonate buffer gives (pH9) for labeling. This solution contains ca. 2mM of labeling reagent.

Warning
 This labeling reagent is deactivated by hydrolysis, especially in alkali solution. The reagent dissolved in water should be used immediately. For temporary storage, the reagent should be dissolved in buffer solution at acidic pH (pH~5) and kept at 0°C.


Please refer to “Anti-DTBTA-Eu3+ Antibody” for the application of the DTBTA-Eu3+ label.

References


参考文献


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