Om uw bezoek aan onze website nog makkelijker en persoonlijker te maken, zetten we cookies (en daarmee vergelijkbare technieken) in. Door verder te gaan op onze website, accepteert u het gebruik van cookies. U kan op elk ogenblik uw cookieinstellingen wijzigen.
Influenza viruses are members of the Orthomyxoviridae family, and as such are enveloped viruses that contain segmented genomes composed of negative-sense, single-stranded RNA1). There are three genera of in?uenza viruses, types A, B and C. Type A in?uenza viruses are grouped into subtypes based on antigenic properties of the viral-encoded hemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins. Monoclonal antibodies are essential tools for many molecular immunology investigations. Especially, anti-in?uenza virus monoclonal antibodies are useful for diagnosis and investigations of virus structure. These monoclonal antibodies introduced here are suitable for use in ELISA and Western blot. ¦Typical Procedure
Figure. Reactivity of the anti-in?uenza A hemagglutinin monoclonal antibody
A. ELISA assay. Each well was coated by 50 ng puri?ed in?uenza hemagglutinin H3. 0-100 ng anti-In?uenza A virus Hemagglutinin H3 monoclonal antibody (TCI Product No. : I0779) was added to the well. After washing, bound antibody was detected using goat-anti-mouse IgG-HRP conjugate. The signal was developed with ABTS substrate (TCI Product No. : A2166). B. Western Blot. Analysis was performed using anti-In?uenza A virus Hemagglutinin H3 monoclonal antibody (I0779) at 1 μg/ml. Goat-anti-mouse-HRP conjugate was used as the secondary antibody. The signal was visualized using DAB-nickel as the chromogen. Molecular weight marker (M). Puri?ed Hemagglutinin (Lane 1).