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Serotonin Metabolites
Serotonin Metabolites
| Column | TCI Dual ODS-CX15 4.6 mmI.D. X 150mm |
|---|---|
| Mobile Phase | A) CH3CN / 50mmol/L HCOONH4(pH3.5,HCOOH) = 10/90 B) CH3CN / 50mmol/L HCOONH4(pH3.5,HCOOH) = 70/30 A to 50% B in 30min |
| Temperature | 25 °C |
| Detection | UV 254 nm |
| Flow Rate | 1.0 mL/min |
| Sample | 1.5-Hydroxy-L-tryptophan 2.5-Hydroxyindole-3-acetic Acid(5-HIAA) 3.Acetylserotonin 4.L-Tryptophan 5.Serotonin(5HT) 6.Melatonin |