CAS RN: | Product Number: B5944
Bovine Serum Albumin Maleimide Conjugate
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Volume/Quantity : 1.0 mg x 3 vial
Conjugation : Purified Bovine Serum Albumin (BSA) was conjugated to Sulfo-SMCC (TCI : code S0883).
Product Form : BSA Maleimide Conjugate.
Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl,
1 mM EDTA, 40 mM sucrose, pH 6.6.
Preservative : None
Store at －20 ºC to －80 ºC (Preferably at －80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
T3531 Streptavidin Maleimide Conjugate
S0883 Sulfo-SMCC Sodium Salt
BSA Maleimide Conjugate is designed to facilitate the conjugation of the BSA to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated BSA contains 15–35 maleimide groups for each BSA molecule. BSA is conjugated to haptens and typically used as a non-relevant carrier in an ELISA for measuring specific anti-hapten antibody titers.
1. Firstly return the vial to room temperature.
2. Reconstitute the contents of the vial with 100 μL of buffer or water to obtain a 10 mg/mL solution of BSA Maleimide.
Note: Do not vortex or heat the BSA Maleimide.
3. Dissolve 1 mg of a thiol-containing molecule in 100-250 μL of buffer or water. (4-10 mg/mL)
In case of thiol-containing molecules insoluble in buffer or water, DMSO may be used for solubilization
(caution : less than 30% DMSO in the final reaction solution). We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 0.1M EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. For complete and efficient conjugation, the BSA Maleimide should mix with excess ratio of thiol-containing molecule. Alternatively, if an excess of thiol-containing molecule is not available, it is better to add a sulfhydryl-containing compound, such as cysteine, for quench remaining active maleimide groups.
4. Immediately mix the thiol-containing molecule and BSA Maleimide solutions and allow them to reactfor 2 hours at room temperature.
5. If desired, desalt, dialyze or purify the conjugate.
|Physical State (20 deg.C)||Solid|
|Appearance||White to Brown to Dark green powder to crystal|
|Molar Ratio Maleimido||15-35 moles of maleimide per mole of BSA|
Safety Data Sheet (SDS)
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C of A & Other Certificates
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