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CAS RN: | Product Number: B5944

Bovine Serum Albumin Maleimide Conjugate

  • BSA Maleimide
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Product Information
Volume/Quantity : 1.0 mg x 3 vial
Conjugation : Purified Bovine Serum Albumin (BSA) was conjugated to Sulfo-SMCC (TCI : code S0883).
Product Form : BSA Maleimide Conjugate.
Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl,
1 mM EDTA, 40 mM sucrose, pH 6.6.
Preservative : None

Store at -20 ºC to -80 ºC (Preferably at -80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

Related Products
T3531 Streptavidin Maleimide Conjugate
S0883 Sulfo-SMCC Sodium Salt

BSA Maleimide Conjugate is designed to facilitate the conjugation of the BSA to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated BSA contains 15–35 maleimide groups for each BSA molecule. BSA is conjugated to haptens and typically used as a non-relevant carrier in an ELISA for measuring specific anti-hapten antibody titers.

1. Firstly return the vial to room temperature.

2. Reconstitute the contents of the vial with 100 μL of buffer or water to obtain a 10 mg/mL solution of BSA Maleimide.
Note: Do not vortex or heat the BSA Maleimide.

3. Dissolve 1 mg of a thiol-containing molecule in 100-250 μL of buffer or water. (4-10 mg/mL)
In case of thiol-containing molecules insoluble in buffer or water, DMSO may be used for solubilization
(caution : less than 30% DMSO in the final reaction solution). We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 0.1M EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. For complete and efficient conjugation, the BSA Maleimide should mix with excess ratio of thiol-containing molecule. Alternatively, if an excess of thiol-containing molecule is not available, it is better to add a sulfhydryl-containing compound, such as cysteine, for quench remaining active maleimide groups.

4. Immediately mix the thiol-containing molecule and BSA Maleimide solutions and allow them to reactfor 2 hours at room temperature.

5. If desired, desalt, dialyze or purify the conjugate.

Product Number B5944
Physical State (20 deg.C) Solid
Storage Temperature -20°C
Appearance White to Brown to Dark green powder to crystal
Molar Ratio Maleimido 15-35 moles of maleimide per mole of BSA
Properties (reference)
Related Laws:
Transport Information:
HS Number 3502909000
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