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CAS RN: 28718-90-3 | Product Number: A2412

2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride [for Biochemical Research]


Purity: >90.0%(HPLC)
Synonyms:
  • 4',6-Diamidino-2-phenylindole Dihydrochloride
  • DAPI·2HCl
  • 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
Documents:
5MG
€38.00
4   7  

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Product Number A2412
Purity / Analysis Method >90.0%(HPLC)
Molecular Formula / Molecular Weight C__1__6H__1__5N__5·2HCl = 350.25  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 28718-90-3
Reaxys Registry Number 4894417
PubChem Substance ID 135727109
MDL Number

MFCD00012681

Specifications
Appearance Light yellow to Yellow to Orange powder to crystal
Purity(HPLC) min. 90.0 area%
DNA staining test to pass test
Properties (reference)
GHS
Related Laws:
EC Number 249-186-7
Transport Information:
HS Number 2933998090
Application
Fluorescence Staining of Cell Nucleus

DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.

A2412

HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.

References


PubMed Literature


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